News
Happy new 2025, a new publication from In2Sight
The publication “A 3D millifluidic model of a dermal perivascular microenvironment on a chip”, by Chiara Martinelli et al., appeared on 6th January 2025 on “Lab on a chip” is the result of a collaboration between polimi and unimib teams of the In2Sight project. Dermal perivascular microenvironment on a chip. The model of the skin is experimentally reproduced in vitro within the miniaturizedoptically accessible bioreactor’s (MOAB), carrying the microstructures used in the In2Sight technology. All details at the publisher link. …
End-of-2024 publication from In2Sight
A publication just appeared on Optics Express as a result of the research ongoing at the Polimi and UNIMIB groups. High dioptric power micro-lenses fabricated by two-photon polymerization. Behjat S. Kariman, Alessandra Nardini, Mario Marini, Pablo Roldán-Varona, Claudio Conci, Manuela T. Raimondi, Roberto Osellame, Giulio Cerullo, Giuseppe Chirico, and Rebeca Martínez Vázquez. Opt. Express 32(27), 48114-48131 (2024) We design, fabricate, and test a single micro-optical element with high dioptric power and high NA (up to 1.25 in water). The dioptric power and magnification of the microlenses were quantified over a 200 × 200 µm aberration-free field of view. Our results indicate that these …
School in Biophysics, Erice, May 2025
Don’t miss in May 2025 the International school on “Shaping light in space and time for biomedical imaging“ Location: Erice, Sicily (I) Applications should be sent by 15 February 2025 through this form. You can download here the leaflet to share with colleagues and students. For any additional information visit this page or contact contacts@in2sight.eu …
New exciting video from IN2SIGHT partners
Unimib and Polimi have recently published video on their activity in IN2SIGHT. You can find them also on our youtube channel …
In2sight presents its results at Photonics Europe, SPIE
In2Sight participated to the European congress “Photonics Europe” in Strasbourg. Presentations were given by Dr. Maria Farsari and Dr. Giuseppe Chirico. …
An exciting new publication from IN2SIGHT in 2024
Published early in 2024, the work on “In vivo label-free tissue histology thorugh a microstructured imaging window” appeared in APL Bioengineering. An exaple of the potentiality of non-linear excitation microscopy in the IN2SIGHT niche to single out different types of cells in vivo without the need of staining. Full reference: In vivo label-free tissue histology through a microstructured imaging window Conci, C., Sironi, L., Jacchetti, E., …Chirico, G., Raimondi, M.T.APL Bioengineering, 2024, 8(1), 016102 …
In2Sight at Focus on Microscopy 2024
In2sight is excited to be at FOM 2024, presenting its results: Mario Marini (unimib) presents the results about “Implantable Micro-Fabricated Lenses for Multiphoton Imaging In Vivo”, M. Marini1, R. Martínez Vázquez, M. Bouzin, L. Giannoni, M. Collini, A. Nardini, B.S. Kariman, C. Conci, E. Kabouraki, K. Tourlouki, N. Kehagias, R. Osellame, M. Farsari, F.S. Pavone, G. Cerullo, M.T. Raimondi, G. Chirico (1Department of Physics, Università degli Studi di Milano-Bicocca, Milan, Italy) We are also present with posters: — Double Phasor Analysis in Digital Pathology: Segmentation and Quantification of Hemochromatosis in Liver Biopsies. D. Panzeri1,E. Pagani,M. Bouzin,L. Presotto,L. D’Alfonso,M. Collini,D. Inverso,G. Chirico,L. Di Tommaso,L. Sironi (1Department of Physics “G. Occhialini”, Università degli Studi di Milano-Bicocca, Piazza della Scienza 3, …
Dissemination to the High schools
We are thrilled to share that In2sight had the pleasure of meeting students from four high schools in the Lombardy area on February 22, 2024. The engagement was part of the program of the University of Milano-Bicocca, focused on incoming orientation for high school students transitioning to their university degree. 🎓 Inspiring the Next Generation In2sight is committed to fostering educational journeys and providing valuable insights to young minds. Our session aimed to guide and inspire students as they navigate the exciting path towards higher education. ⚖️ Achieving Gender Balance We are proud to highlight that gender balance was fully …
In2sight joins 3Rs intiative in europe
In2Sight seeks to advance the creation ethically sustainable bio-tests by introducing a revolutionary in-vivo optical imaging tool with the potential to quantify and analyze the host’s inflammatory response to implants. Form 2022, with distinguished partners from five countries, In2Sight is also part of the Cost Action IMPROVE (CA21139), a pan-European research infrastructure dedicated to expediting the translation of cutting-edge optical imaging tools from the laboratory to clinical practice, and of the 3R Italian center. …
Nov. 2023: In2Sight publish in vivo validation
A publication on APL Bioengineering from In2Sight. “In vivo label-free tissue histology through a microstructured imaging window”, accepted on APL Bioengineering (6 nov 2023). Figure shows the comparison of the H&E staining of tisse within the Atlas to the confocal and two-photon images, to identify red blood cells and granulocytes in the Atlas. …
In2Sight at congresses in 2023
In2Sight has been present at the following congresses in 2023. for a full list please refer to this file. …
Presentation of polimi at EOSAM 2023
Dr. Behjat Sadat Kariman from Politecnico di Milano presented the results of the microfabrication tasks of IN2SIGHT at the European Optical Society annual meeting. Her talk on “Fabrication of Plano-convex Micro-lenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy” was presented on 12 September 2023 at the TOM 3 Biophotonics session of the congress in Dijon (FR). https://x.com/fastgroup_ifn/status/1701965131276525876?s=20 …
New publications from In2Sight Consortium
Two new publications have been recently published from the In2Sight partners from IESL of FORTH. A review on two-photon polymerization(TPL) for Optics and Photonics at Advanced Functional Materials: This work (reachable at this link) reviews the recent progress in the TPL related optical research: it starts with the fundamentals of TPL and material formulation, then discusses novel fabrication methods, and a wide range of optical applications. A research work on high-resolution 3D printed scaffolds for cell studies at Results in Materials: This study (reachable at this link) highlights the possibility to successfully use widely-available, low cost 3dprinters for high-resolution scaffold fabrication while maintaining cell culture compatibility for biomedical applications. …
In2Sight 2-year meeting
It was the mid-term consortium meeting of the project, completing 2 years of running.On the first day, the meeting took place at the Università degli Studi di Milano-Bicocca, where the project coordinator is based.On the second day, we moved to the campus of Politecnico di Milano, where partners from the Department of Chemistry and Physics are located. We had great time with fruitful discussions about the progress of the project and brainstorming.We have also enjoyed the lab tours at the two Universities. Thank you both for hosting us these 2 days! …
New publication in Advanced Functional Materials
Our new open access article was just published in Advanced Functional Materials! “Microlenses Fabricated by Two-Photon Laser Polymerization for Cell Imaging with Non-Linear Excitation Microscopy“ Authored by: M. Marini, A. Nardini, R. Martínez Vázquez, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. S. Kariman, M. Farsari, E. Kabouraki, M. T. Raimondi, G. Chirico This article shows for the first time that it is possible to use the microfabricated lenses to efficiently induce non linear excitation and collect two-photon excitation images from cells with the same level of laser intensity & similar signal to noise ratio. This is the result of a collaborative work from the In2Sight consortium led by Prof. Giuseppe Chirico:🔸 Università degli …
Unimib is hiring
Simulation of immune-reaction to biomaterials. Aim of the project: the objective of this project is to build a digital twin of the inflammatory response and fibrotic process in a foreign body reaction. With such a system validated, it will be possible to monitor continuously the evolution of the reaction by monitoring a limited set of parameters in only few timepoints. Methodology: Different strategies will be compared: ordinary differential equations models and agent based models. Public database and literature searches will be performed to compile all relevant pathways and their reaction rates. Special care will be used to integrate mechano-transduction pathways together …
New publication from In2Sight consortium
we are excited to share our new preprint at bioRxiv!“Microlenses fabricated by two-photon laser polymerisation for intravital cell imaging with non-linear excitation microscopy“ This is the results of more than 6 months of collaborative work from In2Sight partners: socials: Linkedin, twitter …
In2Sight at SPIE Optical Metrology 2023
In2Sight will be present at the Conference OM105: Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI! ➡ Prof. Giberto Chirico is on the Program Committee.➡ Dr. Laura Sironi will chair a special session on “Digital and Computational Pathology”. The intention of this conference is to bring together researchers working in the emerging fields of biomaterials either at microscopic or at macroscopic scale. Note that the registration to SPIE Optical Metrology, grants admission to all conferences held as part of the LASER World of Photonics Congress! …
In2Sight at MNE – Eurosensors 2022
The 48th conference MNE EUROSENSORS 2022 was held in Leuven on 19-23 September 2022. In2Sight partners had a poster presentation and represented by Prof. Giuseppe Chirico and Prof. Laura Sironi. “A novel microstructured implantable window for in-vivo nonlinear excitation imaging”, Prof. Giuseppe Chirico, Dr. Claudio Conci, Prof. Laura Sironi , Dr. Emanuela Jacchetti, Mr. Davide Panzeri, Dr. Donato Inverso, Dr. Rebeca Martinez Vazquez, Prof. Giulio Nicola Cerullo, Dr. Roberto Osellame, Prof. Maddalena Collini, Dr. Maria Farsari, Dr. Elmina Kabouraki, Prof. Manuela Raimondi. …
In2Sight presence at EUSAAT congress 2022
The 2022 European Congress on Alternatives to Animal Testing (EUSAAT) took place at the University of Linz in Austria on 26-28 September 2022. In2Sight had an exhibition desk and represented by: Prof. Giuseppe Chirico from Università degli Studi di Milano-Bicocca Dr. Maria Kitsara from PRAXI Network / Foundation for Research and Technology – Hellas (FORTH) Dr. Javier Martínez de Velasco from ASPHALION Our partners had the opportunity to discuss with the EUSAAT community, while Prof. Chirico gave an oral talk on “An in-vivo engineered #chip as a smart intravital multiphoton imaging window for new validation protocols of biomaterials”. …
Photonics meets Biology
The VI Summer School and Workshop ‘Photonics Meets Biology‘ took place at the beautiful island of Spetses in Greece on 27th July 27th – 1st August 2022. The event was organized by the In2Sight partner, Dr. Maria Farsari, who is leading the group of “Light-based Additive Manufacturing” at IESL of FORTH. Prof. Giuseppe Chirico, from the University of Milano Bicocca, gave a talk on “In to sight: a laser fabricated window for in-vivo imaging”. …
In2Sight at International School of Nanomedicine
Erice, Sicily, 20-24 July 2022 The 5th workshop on “Advanced Nano- and Microstructured Materials for Medical Applications” was held in Erice (Sicily), on 20-25 July 2022. The workshop was organized by the International School of Nanomedicine at the Ettore Majorana Foundation and Centre for Scientific Culture. Prof. Giuseppe Chirico from the University of Milano Bicocca, codirected the workshop and gave a lecture on “Laser Microfabrication for implantable devices”. …
European Congress on Alternatives to Animal Testing 2022
From September 26 – 28 at the University of Linz, Austria IN2SIGHT will be present at the 2022 Congress of the Eusaat society with a presentation1 and a desk throughout the congress. The congress will take place in Linz, in September 2022. “An in-vivo engineered chip as a smart intravital multiphoton imaging window for new validation protocols of biomaterials“, G. Chirico …
Workshop “Innovation for biocompatibility evaluation”, held on May 12, 2022
On May 12th 2022 the workshop on “Innovation for biocompatibility evaluation” was successfully held both physically at University of Milano-Bicocca and online. The workshop was organized in the framework of the H2020 FET project In2Sight. Prof. Arti Devi Ahluwaia from Center 3R and University of Pisa gave an insightful talk on “The 3rs in Italy. Where we are.” She pointed out that “We need to push the acknowledgment of the principle of the 3R’s in teaching as well in research to educate a new class of researchers“. “Our vision is to make people understand that the 3R’s (refine, reduce and …
Join the PhD in Physics @UNIMIB
Projects for PhD candidates in Physics within the IN2SIGHT project are avaliable on Adaptive optics correction of tissue scattering for in-vivo optical imaging in tissues Artificial Intelligence methods for data clustering in digital pathology Please contact In2Sight for details. The call for applications to the Ph.D. programmes – a.y. 2022/2023 is online Deadline: 20th May 2022 at noon. Information at the Unimib website. …
Photonics meets biology, July 2022
VI ‘Photonics Meets Biology’, Summer School and Workshop Summer School: 27-29 July 2022 Workshop: 30 July -1 August 2022. Where: Spetes Island (GR) Visit the website here …
5th Workshop: “Microstructured materials for medical applications” (co-organized by in2sight)
The workshop is co-organized by the International School of Nanomedicine at the Ettore Majorana Foundation and Center for Scientific Culture, ERICE (Sicily) and IN2SIGHT. For further information and applications, visit the website here. …
Workshop: “Innovation for biocompatibility evaluation” (online and in person @Unimib)
12 May 2022: Academy meets industry and regulatory bodies to discuss of alternative solutions for biocompatibility tests. A workshop in hybrid modality (in person and online) with panel session. Date: 12 May 2022; Location: Milano-Bicocca, room U6-26 and streaming on webex/unimib. please NOTICE the change of the room number: room U6-26 (building U6, first floor) Event free of charge, registration needed: register HERE.For additional info, email to: contacts@in2sight.euEvent poster …
News from POLIMI
NEW ENTRY AT THE MECHANOBIOLOGY LAB! May 16, 2021 – Dr. Chiara Martinelli started her appointment today at the Mechanobiology lab. She will work on the FET Open project IN2SIGHT to develop an in vivo bioengineered chip as a smart intravital multiphoton imaging window for new validation protocols of biomaterials. Her research is funded under the European Commission program Horizon Europe. Congratulations to Chiara and welcome at the spectacular IN2SIGHT partner team of the Mechanobiology lab to our first full-time molecular biologist, yea! PHD FELLOWSHIP AWARDED TO ALESSANDRA NARDINI April 9, 2021 – Alessandra Nardini today was awarded a fellowship …
Latest news from In2Sight
We consider all the drivers of change – from the ground up and we’ll motivate and support you to make the change. …
News from Università Milano-Bicocca
We consider all the drivers of change – from the ground up and we’ll motivate and support you to make the change. …
News from DFKZ
We consider all the drivers of change – from the ground up and we’ll motivate and support you to make the change. …
Manuela Raimondi is full professor of Bioengineering at Politecnico di Milano since 2009.
